October 4, 2017


Although the bacterial topoisomerases gyrase and topoisomerase IV are critical for cell function and are targets for quinolone antibacterials (such as Cipro), little is known about how these enzymes remove positive supercoils on overwound DNA. Neil Osheroff, Ph.D., and colleagues report in Nucleic Acids Research that gyrase removes positive supercoils rapidly and uses a DNA wrapping mechanism to unwind DNA.