Alan R. Brash, Ph.D.

Alan R. Brash, Ph.D.

Professor, Department of Pharmacology

(615) 343-4495
(615) 322-4707

Techniques used in the Brash lab:

We are specialists in the analytical biochemistry of fatty acid oxygenases. To set up the experiments and analyze the fatty acid products we routinely do the following: clone and express enzymes; site-directed mutagenesis; protein purification; synthesis of fatty acid analogs; analysis of enzyme reactions; westerns and other gels; HPLC analysis of enzymic products; UV-Visible spectroscopy; mass spectrometry (GC-MS and LC-MS); NMR for structural analysis of novel substrates and products.

 

Projects:

Role of lipoxygenases in skin barrier function

Mechanism of biosynthesis of lipoxygenase products

Catalase-related hemoproteins that metabolize fatty acid hydroperoxides