Publications

2015

Newcomer ME, Brash AR. (2015) The structural basis for specificity in lipoxygenase catalysis. Protein Sci. 24(3):298-309 PMCID:PMC4353356

Mashhadi Z, Boeglin WE, Brash AR. (2015) Robust inhibitory effects of conjugated linolenic acids on a cyclooxygenase-related linoleate 10S-dioxygenase: Comparison with COX-1 and COX-2. Biochim Biophys Acta. 2015 Oct;1851(10):1346-52. PMCID: PMC4561586

Teder T, Lõhelaid H, Boeglin WE, Calcutt WM, Brash AR, Samel N. (2015) A Catalase-related Hemoprotein in Coral Is Specialized for Synthesis of Short-chain Aldehydes: DISCOVERY OF P450-TYPE HYDROPEROXIDE LYASE ACTIVITY IN A CATALASE.

J Biol Chem. 2015 Aug 7;290(32):19823-32. PMCID: PMC4528142

 

2014

Brash AR, Niraula NP, Boeglin WE, Mashhadi Z. (2014) An Ancient Relative of Cyclooxygenase in Cyanobacteria Is a Linoleate 10S-Dioxygenase That Works in Tandem with a Catalase-related Protein with Specific 10S-Hydroperoxide Lyase Activity. J Biol Chem. 289(19):13101-11 PMCID: PMC4036322

Mashhadi Z, Boeglin WE, Brash AR. (2014) Inhibitory effects of a novel Val to Thr mutation on the distal heme of human catalase. Biochimie. 2014 Nov;106:180-3. PMCID: PMC4250446

Neau DB, Bender G, Boeglin WE, Bartlett SG, Brash AR, Newcomer ME. (2014) Crystal Structure of a lipoxygenase in complex with substrate: the arachidonic acid binding site of 8R-lipoxygenase. J. Biol. Chem. 289(46):31905-13 PMCID: PMC4231669

Teder T, Boeglin WE, Brash AR. (2014) Lipoxygenase-catalyzed transformation of epoxy fatty acids to hydroxy-endoperoxides: a potential P450 and lipoxygenase interaction. J Lipid Res. 55(12):2587-96. PMCID: PMC4242451

 

2013

Jin J., Zheng Y., Boeglin W.E., Brash A.R. (2013) Biosynthesis, Isolation, and NMR Analysis of Leukotriene A Epoxides: Substrate Chirality as a Determinant of the cis or trans Epoxide Configuration. J. Lipid Res. 54(3):754-61. PMCID: PMC3719183

Thomas, C.P., Boeglin, W.E., Garcia-Diaz, Y., O’Donnell, V.B. and Brash, A.R. (2013) Steric analysis of epoxyalcohol and trihydroxy derivatives of 9-hydroperoxy-linoleic acid from hematin and enzymatic synthesis. Chem. Phys. Lipids 167-168: 21-32. PMCID: PMC3678760

Brash, A. R., Boeglin, W. E., Stec, D. F., Voehler, M., Schneider, C., and Cha, J. K. (2013) Isolation and characterization of two geometric allene oxide isomers synthesized from 9S-hydroperoxy-linoleic acid by P450 CYP74C3: Stereochemical assignment of natural fatty acid allene oxides. J. Biol. Chem. 288: 20979-20806. PMCID: PMC3774351

Muñoz-Garcia, A., Thomas, C.P., Keeney, D.S., Zheng, Y., and Brash, A.R. (2013) Importance of the lipoxygenase-hepoxilin pathway in the mammalian epidermal barrier. Biochim. Biophys. Acta 1841(3):401-408 PMCID: PMC4116325

 

 

2012

218. Brash AR, Schneider C, Hamberg M. (2012) Applications of Stereospecifically-Labeled Fatty Acids in Oxygenase and Desaturase Biochemistry. Lipids. 47, 101-116.

    PMCID: PMC3315059

Jin, J., Boeglin, W.E., Cha, J.K., Brash, A.R. (2012) 8R-Lipoxygenase-catalyzed synthesis of a prominent cis-epoxyalcohol from dihomo-γ-linolenic acid: a distinctive transformation compared with S-lipoxygenases. J Lipid Res. 2012 Feb;53(2):292-9. Epub 2011 Dec 10. PMCID: PMC3269157

Gilbert NC, Rui Z, Neau DB, Waight MT, Bartlett SG, Boeglin WE, Brash AR, Newcomer ME. (2012) Conversion of human 5-lipoxygenase to a 15-lipoxygenase by a point mutation to mimic phosphorylation at Serine-663. FASEB J. 26(8):3222-9 PMCID: PMC3405276

Boeglin, W.E., and Brash, A.R. (2012) Cytochrome P450-type hydroxylation and epoxidation in a tyrosine-liganded hemoprotein, catalase-related allene oxide synthase. J. Biol. Chem. 287: 24139-24147 PMCID: PMC3397841

 

2011

Liu, W., Porter, N.A., Schneider, C., Brash, A.R., Yin, H. (2011) Formation of 4-hydroxynonenal from cardiolipin oxidation: intramolecular peroxyl radical addition and decomposition. Free Rad. Biol. Med. 50(1):166-178

Gilbert, N.C., Bartlett, S.G., Waight, M.T., Neau, D.B., Boeglin, W.E., Brash, A.R., Newcomer M.E. (2011) The structure of human 5-lipoxygenase. Science 331: 217-219.

Zheng Y, Yin H, Boeglin WE, Elias PM, Crumrine D, Beier DR, Brash AR. (2011) Lipoxygenases mediate the effect of essential fatty acid in skin barrier formation: A proposed role in releasing omega-hydroxyceramide for construction of the corneocyte lipid envelope. J. Biol. Chem. 286(27):24046-56.

Brash AR, Schneider C, Hamberg M. (2011) Applications of Stereospecifically-Labeled Fatty Acids in Oxygenase and Desaturase Biochemistry. Lipids. 2011 Oct 5. [Epub ahead of print]

    PMID: 21971646

Bandara DM, Sono M, Bruce GS, Brash AR, Dawson JH. (2011) Coordination modes of tyrosinate-ligated catalase-type heme enzymes: Magnetic circular dichroism studies of Plexaura homomalla allene oxide synthase, Mycobacterium avium ssp. paratuberculosis protein-2744c, and bovine liver catalase in their ferric and ferrous states. J. Inorg. Biochem. 2011 Sep 22. [Epub ahead of print] PMID: 22104301

Jin, J., Boeglin, W.E., Cha, J.K., Brash, A.R. (2012) 8R-Lipoxygenase-catalyzed synthesis of a prominent cis-epoxyalcohol from dihomo-γ-linolenic acid: a distinctive transformation compared with S-lipoxygenases. J Lipid Res. 2012 Feb;53(2):292-9. Epub 2011 Dec 10. PMID: 22158855

 

2010

Gao, B., Boeglin, W.E., and Brash, AR (2010) Omega-3 fatty acids are oxygenated at the n-7 carbon by the lipoxygenase domain of a fusion protein in the cyanobacterium Acaryochloris marina. Biochim. Biophys. Acta 2010 Jan;1801(1):58-63. Epub 2009 Sep 25.

Zheng, Y., and Brash, A.R. (2010) Formation of a cyclopropyl epoxide via a Leukotriene A synthase-related pathway in an anaerobic reaction of soybean lipoxygenase-1 with 15S-hydroperoxyeicosatetraenoic acid: Evidence that oxygen access is a determinant of secondary reactions with fatty acid hydroperoxides.  J. Biol. Chem. 285(18):13427-36.

Zheng, Y., and Brash, A. R. (2010) Dioxygenase activity of epidermal lipoxygenase-3 unveiled: Typical and atypical features of its catalytic activity with natural and synthetic polyunsaturated fatty acids. J. Biol. Chem. 285: 39866-39875

Zheng, Y., and Brash, A. R. (2010) On the role of molecular oxygen in lipoxygenase activation: comparison and contrast of epidermal lipoxygenase-3 with soybean lipoxygenase-1. J. Biol. Chem. 285(51): 39876-39887.