Postdoctoral fellowship available in Division of Cardiovascular Medicine (VUMC) for a computational biologist with a terminal degree trained in molecular dynamics of proteins and lipids.
MD simulations will be used with other computational approaches, such as homology modeling and double state normal mode analysis, to produce molecular models for a number of plasma lipoprotein-related proteins: ATP-binding cassette transporter A1 (ABCA1), ATP-binding cassette transporter A4 (ABCA4), paraoxonase 1 (PON-1), monomeric lipid-free and lipid-poor apolipoprotein A-I (apoA-I), apolipoprotein A-II (apoA-II), lecithin cholesterol acyl transferase (LCAT) and phospholipid transfer protein (PLTP).
This position will be funded by a multi-institutional Program Project (PPG) entitled, “Multidisciplinary Approaches to HDL Structure, Assembly and Function” whose central hypothesis is that the platform high density lipoprotein protein, apolipoprotein A-I (apoA-I), is a conformationally dynamic scaffold that facilitates interactions among other HDL proteins and lipid remodeling factors that exert potent biological effects on the artery wall. In collaboration with the other projects and cores of our PPG, these models will be tested with more wet experimental approaches: MSXL, site-directed mutagenesis, functional assays and high resolution electron microscopy. This PPG will use three integrated innovative methods: i) computational simulation and molecular modeling, ii) chemical crosslinking and site-directed mutagenesis of apoA-I and its partner proteins, iii) structure-function probing with mass spectrometric and cell-based assays that will explore the clinical impact of HDL composition and test new ways of assessing HDL functionality.
Applicants should send a cover letter with a description of past research experience, curriculum vitae, a statement of research interests and career goals, and the names of three references to: Dr. Jere Segrest, E-mail: firstname.lastname@example.org.