Resources for chemiluminescent western blotting
Choosing your reagents can be sometimes be difficult when you are developing a procedure for the first time. And unfortunately, there is not a "one size fits all" reagent that everyone thinks is the best. When asked, a particular lab's choice of chemiluminescent reagents can admittedly be based on what has worked by others in the past and not necessarily what works best now. Change can be hard and it is definitely additional work for research staff with full schedules to optimize their assays.
For help in finding the best reagent for you or for more information on chemiluminescent western blotting, please select the links below.
ThermoScientific/Pierce’s “ECL PLUS, Pico, Femto” Reagents
GE Healthcare/Amersham’s “ECL Prime” Reagent
BioRad’s “Clarity” Reagent