Chemiluminescent Imaging

Resources for chemiluminescent western blotting

Choosing your reagents can be sometimes be difficult when you are developing a procedure for the first time. And unfortunately, there is not a "one size fits all" reagent that everyone thinks is the best. When asked, a particular lab's choice of chemiluminescent reagents can admittedly be based on what has worked by others in the past and not necessarily what works best now. Change can be hard and it is definitely additional work for research staff with full schedules to optimize their assays.

For help in finding the best reagent for you or for more information on chemiluminescent western blotting, please select the links below.


UC Davis


Advantages, Disadvantages, and Properties of Nitrocellulose vs. PVDF


ThermoScientific/Pierce’s “ECL PLUS, Pico, Femto” Reagents

Chemiluminescent Western Blotting

Western Blot Chemiluminescent Substrate Selection Guide


GE Healthcare/Amersham’s “ECL Prime” Reagent

Amersham ECL Prime Western Blotting Detection Reagent

Hybond, Optitran, and Protran nitrocellulose blotting membranes

Hybond and Westran PVDF blotting membranes


BioRad’s “Clarity” Reagent

Clarity™ Western ECL Substrate

083013_BioRad_Clarity_Substrate Manual.pdf

BioRad's PVDF, low fluorescing PVDF, and nitrocellulose membranes


Lumigen’s “PS-3, TMA-6, and TMA-3” Reagents

Background on chemiluminescence

Lumigen ECL Reagents