*Note: We have a 2nd floor model Superspeed centrifuge. The Sorvall RC-5B is located in 4100B MRB3.
We have three rotors which can be used in these centrifuges. They are located in the coldroom nearby (3143 MRB3).
The most important thing to remember while using this model of centrifuge is to attach the lid to the rotor OUTSIDE of the centrifuge and then tighten the rotor to the drive shaft. Not doing this can lead to catastrophic failure.
REGULAR CLEANING OF SPILLED LIQUIDS
Metal corrosion can be avoided by followinga routine maintenance program after eachrotor use:
– Clean rotors, lids, adapters and anyassociated parts with a 1% solution ofa mild non-alkaline detergent such asdishwashing liquid, rinse with distilledwater and dry thoroughly with a soft cloth
– Do not use strong alkaline laboratorydetergent on aluminum rotors; if encrustedmaterial ispresent, remove it with asoft, twisted-bristle brush and the1% non-alkaline soap solution
DECONTAMINATION OF BIOHAZARD-CONTAINING LIQUID
Given the nature of samples processedin a rotor, biological or radioactivecontamination is possible. For biological contamination of rotors, a 2% glutaraldehyde solution, ethylene oxide or ultraviolet radiation are the recommended methods of sterilization, while for a rotor that may be contaminated by a radioactive sample, use a solution of equal parts of 70%ethanol, 10%SDS and water. In addition:
– Do not use chlorine bleach on aluminum rotors
– When autoclaving, rotor components should be separated
– If sterilization is not necessary, a 70% solution of ethanol can be used
– Most commercially available detergents for radioisotopic contamination are not compatible with aluminum or anodized coatings and should not be used
– Rinse with ethanol, followed by water and dry with a soft cloth
– Do not immerse Thermo Scientific Fiberlite rotors; spin rotor to remove liquid
– Fiberlite® composite rotors are not compatible with ethylene oxide