- Pfaltzgraff ER, Roth GM, Miller PM, Gintzig AG, Ohi R, Bader DM. Loss of CENP-F Results in Distinct Microtubule Related Defects Without Chromosomal Abnormalities. Molecular biology of the cell. 2016 May 4. PMID: 27146114 [PubMed].
Microtubule (MT) binding Centromere Protein F (CENP-F) has previously been demonstrated to play a role exclusively in chromosome segregation during cellular division. Many cell models of CENP-F depletion show a lag in the cell cycle and aneuploidy. Here, using our novel genetic deletion model, we show that CENP-F also regulates a broader range of cellular functions outside of cell division. We characterized CENP-F(+/+) and CENP-F(-/-) mouse embryonic fibroblasts (MEFs) and found drastic differences in multiple cellular functions during interphase including cell migration, focal adhesion dynamics, and primary cilia formation. Ultimately, we discovered that CENP-F(-/-) MEFs had severely diminished MT dynamics which underlie the phenotypes we describe. These data, combined with recent biochemical research demonstrating the strong binding of CENP-F to the MT network, uphold the conclusion that CENP-F is a powerful regulator of MT dynamics during interphase impacting heterogeneous cell functions.