Why Use This Microscope?
The Nikon PRIMO is a Nikon Ti-2 microscope with widefield fluorescence and an Alveole PRIMO attachment. The PRIMO is a micropatterning, hydrogel polymerization and microfabrication device that allows you to create custom made in vitro cellular microenvironments. It uses a digital micromirror device (DMD) technology that illuminates a pattern with UV light at 375nm.The Nikon Ti-2 is equipped with the D-LEDI fluorescent LED light source and Prime BSI Express Scientific CMOS (sCMOS) camera. The PRIMO also has a white LED light source and Nikon DS-Ri-2 color camera for bright field imaging of histology stained samples. The microscope offers a 22mm FOV image.

Camera:

• Prime BSI Express Scientific CMOS (sCMOS) camera
• Nikon DS-Ri2 color camera

Imaging Modes:

• PRIMO – micropatterning, hydrogel polymerization, and microfabrication
• Chromogenic microscopy (DAB, H&E, etc)
• Conventional fluorescence microscope

Excitation Specs:

• Nikon D-LEDI LED light engine:
  • 385 nm (DAPI)
  • 475 nm (FITC. GFP)
  • 550 nm (TRITC, Cy3)
  • 621 nm (far red, Cy5)

Objective Lenses:

• 4x Plan Fluor NA 0.13 WD 17.1mm
• 10x Plan Fluor DIC N1 NA 0.3 WD 16mm
• 20x Plan Apo λD OFN25 DIC N2 NA 0.8 WD 800um
• 20x S Plan Fluor ELWD DIC N1 NA 0.45 WD 8.2mm (for PRIMO)
• 40x Plan Fluor Oil DIC H N2 NA 1.3 WD 240um

Charges:

Note: To use the microscope you must have an active iLab account. Reservations are made through our on-line iLab calendar. Contact Max, or Tegy to arrange training.

Location:

• MCN T-2216