Zeiss LSM 510
Why Use This Microscope?
This microscope is well suited for viewing samples on sealed, inverted slides or in 35mm dishes with a #1.5 coverglass (Matek) compatible with the inverted format. All slides MUST be sealed, clean and dry prior to placing on the stage.
Imaging Modes:
Laser scanning fluorescence and DIC (Nomarski)
3-D “Z-series”
Time-series
Fluorescence Filters: (excitation/emission)
Blue/Green (457,488,514nm for blue, green, yellow dyes, e.g., CFP,YFP,GFP, Cy-2, FITC-like dyes)
Green/Red (543nm for Cy-3, Texas Red, or rhodamine-like dyes)
Red/Far-Red (633 nm for far-red dyes like Cy-5)
Objective Lenses:
5x/.30 Plan-Neofluar
10x / 0.30 Plan Neofluar
20x / 0.75 Plan-Apochromat
40x / 1.30 Plan-NEOFLUAR OIL
63x / 1.40 Plan-APOCHROMAT OIL
100x / 1.30 Plan-NEOFLUAR OIL
Location:
MRBIII 4155
Charges:
Note: To use the microscope you must have an active iLab Account. Reservations are made through our on-line iLab calendar. Contact Sean to arrange training.
Download / View Quickstart Guide.
| SERVICE | *RATE/UNIT |
|---|---|
| LSM 510 Confocals, scope time | $48.51 / hour |
| Confocal, scope time scope time | $52.14 / hour |
| Widefield(Non-COE), scope time | $48.51 / hour |
| TEM, scope time | $118.00 / hour |
| Zeiss Crossbeam, scope time | $64.92 / hour |
| Live Imaging, Extended Hours (special permissions) | $36.62 / hour |
| Nikon Spinning Disk, scope time | $36.62 / hour |
| Nikon SoRa, scope time | $36.62 / hour |
| Nikon SIM Super Resolution, scope time | $36.62 / hour |
| Nikon STORM Super Resolution, scope time | $36.62 / hour |
| Nikon TIRF, Multi-Excitation | 36.62 / hour |
| Zeiss Z.1 Lightsheet | $36.62 / hour |
| EM PROCESSING | |
| Embed (Spurr's) | $61.13 / capsule |
| Embed Durcupan | $61.13 / capsule |
| Embed SBEM | $118.07 / capsule |
| Freeze Substitution | $118.07/ capsule |
| High Pressure Freezer | $36.44 / capsule |
| Thick section | $62.04 / capsule |
| Thin section | $121.69 / capsule |
| Negative stain, material only | $6.00 / hour |
| ESEM prep, material only | $10.00 / hour |
| Sputter Coater | $10.00 / hour |
| CPD | $10.00 / hour |
| Technical Help | |
| Imaging for hire, training, protracted help | $69.55 / hour |
| Analysis Workstations | $9.01 / hour |