Why Use This Microscope?
The N-SIM structured illumination platform is a super-resolution microscope capable of approximately doubling the resolving capability of traditional fluorescence microscopy. Unlike “iterative” super-resolution techniques such as STORM and PaLM, SIM does not require stringent sample preparation or imaging buffers and can be used with most standard fluorescent proteins and/or organic dyes. Our system is outfitted to accomplish 2D- and 3D- SIM on both static and fixed samples and TIRF-SIM for live preparations. Importantly, thanks to its integration with a high-end workstation and robust GPU, the lengthy SIM reconstruction processes of the past have been dramatically revamped to enable near real-time reconstruction and visualization of the contribution of parameter modification “on-the-fly”. Such advancements have speed up the SIM imaging process nearly 12 fold.

Camera:

• Andor iXon Ultra DU-897 EMCCD monochrome camera

Imaging Modes:

• 2D SIM (multi- channel, -plane) live or fixed samples
• 3D SIM (multi- channel, -plane) live or fixed samples
• TIRF SIM (currently single channel) live samples

Excitation Specs:

• Full set of visual cubes for blue, green, and red –emitting probes.
• Diode Laser Lines and Power (measured at fiber tip):
  • 405nm, 21 mW
  • 488nm, 74 mW
  • 561nm, 78 mW
  • 647nm, 42.5 mW

Objective Lenses:

• SR Apo TIRF (oil) 100x 1.49 NA WD 0.12

Charges:

Note: To use the microscope you must have an active iLab account. Reservations are made through our on-line iLab calendar.Contact Max or Tegy to arrange training.

Location:

• MCN T-2216