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Targeted multiplex imaging mass spectrometry with single chain fragment variable (scfv) recombinant antibodies.


Thiery| Mernaugh| Yan| Spraggins| Yang| Parl| Caprioli G| RL| H| JM| J| FF| RM , . Journal of the American Society for Mass Spectrometry. 2012 10 ; 23(10). 1689-96


Recombinant scfv antibodies specific for CYP1A1 and CYP1B1 P450 enzymes were combined with targeted imaging mass spectrometry to simultaneously detect the P450 enzymes present in archived, paraffin-embedded, human breast cancer tissue sections. By using CYP1A1 and CYP1B1 specific scfv, each coupled to a unique reporter molecule (i.e., a mass tag) it was possible to simultaneously detect multiple antigens within a single tissue sample with high sensitivity and specificity using mass spectrometry. The capability of imaging multiple antigens at the same time is a significant advance that overcomes technical barriers encountered when using present day approaches to develop assays that can simultaneously detect more than a single antigen in the same tissue sample.


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