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Laser Ablation Inductively Coupled Plasma IMS

We developed a LA source to increase sensitivity (by reducing sample loss during transport to the MS) and facilitate LA-ICP imaging (Figure 1). This technology has been applied to imaging infectious diseases to gain an understanding of metal trafficking when a pathogen is within a host [1].  This capability is part of the infrastructure of the MSRC and is available for use by the Resource for driving biology projects and collaborations.


Figure 1. LA source for LA-ICP IMS. The redesigned geometry provides complete capture of the ablated plume, greatly reduces the loss of ablated atoms during transport to the ICP-MS, and reduces the potential for sample contamination by previously ablated material. Black arrows indicate the direction of carrier gas flow. Distance from ablation site to ICP plasma can be over 30 cm for standard geometry LA sources but is only 12 mm for the redesigned source.


LA-ICP MS: 1R21 AI107233


1. Kehl-Fie TE, Zhang Y, Moore JL, Farrand AJ, Hood MI, Rathi S, Chazin WJ, Caprioli RM, Skaar EP (2013) MntABC and MntH contribute to systemic Staphylococcus aureus infection by competing with calprotectin for nutrient manganese. Infect Immun 81 (9):3395-3405. PMCID: 3754211. Return to text.