Modification of the Cell Cycle by 4-hydroxynonenal (HNE)
4-hydroxynonenal (HNE) is a product of lipid peroxidation that occurs as a result of high levels of oxidative stress. My work focuses on the covalent modification of proteins by HNE that can alter protein function resulting in the dysregulation of cellular pathways. Through previous mass spectrometry analysis, our group has shown that HNE can covalently modify CDK2, a main cell cycle regulator in the G1/S transition. The goal of my project is to determine how modification of CDK2 by HNE results in alteration of the cell cycle.