The Delpire laboratory investigates mechanisms of cell volume regulation, intracellular chloride homeostasis, and epithelial sodium transport. This involves studying the function of electroneutral cation-chloride cotransporters such as NKCC1-2 (Na+-K+-2Cl- cotransporters) and KCC1-4 (K+-Cl- cotransporters). I currently investigate an isoform of the Na+-K+-2Cl- cotransporter, known as NKCC1, which is ubiquitously expressed throughout the body. Specifically, I utilize CRISPR technologies to inject truncated NKCC1 in rodents and perform flux assays on Xenopus Laevis oocytes to serve as a model for disease, in order to better understand human NKCC1 mutations and potentially providing an avenue for novel drug therapies.
PI: Eric Delpire, Ph.D.