- Keck VA, Edgerton DS, Hajizadeh S, Swift LL, Dupont WD, Lawrence C, Boyd KL. Effects of Habitat Complexity on Pair-Housed Zebrafish. Journal of the American Association for Laboratory Animal Science : JAALAS. 54(4). 378-83. PMID: 26224437 [PubMed].
Sexually mature zebrafish were housed as single male-female pairs with or without plastic vegetation for 1, 5, or 10 d for comparison of whole-body cortisol measured by radioimmunoassay. Individually housed male zebrafish were used as controls. In the fish that were pair-housed without vegetation (NVeg), one animal died in 5 of 24 pairs, and one animal was alive but wounded in an additional pair. No deaths or wounds occurred in the fish that were pair-housed with vegetation (Veg). Cortisol levels did not differ between the treatment groups on day 1. On day 5, cortisol values were higher in the Veg group than in the individually housed fish (P