The Histology Lab is located in Medical Center North and supports the research efforts of orthopaedic surgeons, neurosurgeons, their residents, bone biologists, as well as both Chemical and Biomedical Engineering students and faculty. To accommodate the wide variety of research projects, the lab is equipped with state-of-the-art machinery and software.
Histology Lab Team
Jeffry Nyman, PhD
Assistant Professor of Orthopaedic Surgery and Rehabilitation, Biomedial Engineering
Daniel S. Perrien, PhD
Research Assistant Professor of Orthopaedic Surgery and Rehabilitation
Sasidhar Uppuganti, MS
Biomedical Engineer II
Histology Lab Equipment
Specimen Preparation Tools:
The orthopaedic histology lab houses a variety of tools for embedded specimen’s mounting, sectioning, and preparation. These tools also aid handling of non-embedded bone samples to be prepared for further use in biomechanics and Micro-CT imaging.
EXAKT 310 Diamond Band Saw
The Exakt system is used to produce thick sections of resin-embedded tissues containing ceramic and metal implants for microscopic examination. It has the ability to provide clean, plane-parallel cuts with minimal burrs. This system is also used to support numerous preclinical and translational studies such as:
• those aimed at developing new materials for musculoskeletal tissue regeneration
• hardware for fracture stabilization or joint fusion
• ceramic bone void fillers
• It can also be used to examine clinical specimens retrieved during joint replacement or other hardware revision surgeries.
EXAKT 400CS Grinding System
The EXAKT 400CS plate grinder system has the ability to create thin ground sections with plane-parallel and outstanding surface quality. Sample thicknesses in the range of 20 µm can be achieved easily. The deviation of the sample thickness after the grinding lies within a precision of approx. +/- 1.5 µm. It is equipped with the AW110 controller that can have force, speed presets making it easy to process a variety of materials and hard/soft combinations.
BUEHLER VibroMet 2 Vibratory Polisher
This polishing tool is used to prepare high quality polished surfaces on a wide variety of materials, including EBSD applications. The 7200 cycles per minute horizontal motion produces a very effective polishing action, providing superior results, exceptional flatness and less deformation. A 12in [305mm] bowl size helps accommodate several samples simultaneously.
The facility also houses a standard recirculating laboratory fume hood to aid in sample preparation stages such as embedding or potting.