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VAPR – Custom Antibody, Nanobody, and Recombinant Protein Experts

A division of the Vanderbilt Institute of Chemical Biology (VICB)

VAPR partners with scientists across the country to design, develop, and evaluate all the molecular tools and assays needed to drive research forward.

Our broad experience and deep expertise allow us to pursue the most challenging protein and antibody projects. We work on a wide spectrum of projects from basic reagent development, to diagnostic assays, to large-molecule therapeutic generation and assessment. Click on any service below to learn more.

Nanobody Production

  • Novel protein conformation determination based on specific nanobody generation
    • Crystallization chaperones
    • Modulation of activity (PAMs or NAMs)
  • Intracellular compartment localization once nanobody labeled
    • Non-invasive imaging
  • Nanobodies can be used in a pull-down assay
    • Affinity capture reagent
  • Block protein binding and enzymatic mechanisms
    • Disrupt protein:protein interactions
  • Used in ELISA and other protein assays
  • Western/IHC
  • Therapeutics
  • Spatiotemporal dynamics of endogenous protein

Monoclonal and Polyclonal Antibody Services

  • Monoclonal antibody purification from existing hybridomas to fit any scale or budget
  • Antibody labeling
  • Antigen generation and purification
  • Hybridoma optimization
  • Polyclonal Rabbit antibody production with Cocalico
  • Polyclonal testing and purification

Recombinant Antibody / Antibody Engineering Services

  • Isolation and sequencing of antibody variable (VH and VL) domains from hybridomas
  • Small and large-scale antibody productions/purifications from cloned antibodies
  • Antibody humanization (chimera generation)
  • Single-chain (scFv) generation
  • Antibody Optimization

Recombinant Protein Services

  • Recombinant protein expression, optimization, and purification
  • Codon optimization of the target for maximum expression
  • Mammalian cell-based expression (transient and stable)
  • Kinetic and Quantitative Analyses of Protein:Protein and Protein:Small Molecule Interactions

Assay and Labeling Services

  • Sandwich and Direct ELISA assay development
  • Initial antibody pairing and standard validation
  • Assay qualification
  • ELISA testing service with custom or commercial assays
  • Services to perform most antibody-based assays
  • Protein and Antibody labeling/conjugation
  • Any scale
  • Carriers, Resins, Magnetic beads, etc
  • Fluorophores, CyTOF labels, Biotin, etc
  • mRNA testing

General Purpose Antibodies, Enzymes, and Other Reagents

  • VAPR offers extremely high-quality purified antibodies and molecular biology enzymes to Vanderbilt Investigators.
  • Fully Functional Signaling Molecules (Ex. Wnt, R-spondin).
  • Molecular Biology Reagents and kits (Ex. Quick pour PAGE Gels, Plasmid DNA purification kits).
  • Quality control tested and 100% guaranteed.  To see the full list of available reagents go to the repositories web page.