Custom Monoclonal Services
Our custom rodent monoclonal project process is designed to produce novel antibodies against proteins, peptides, or other antigenic targets. We work closely with each customer to maximize the success rate for obtaining antibodies that perform in the assays of interest to the investigator.
Project time lines vary depending on the response of animals to the particular antigen. Our most important goal is to not enter into the more expensive and labor-intensive hybridoma generation stage until the chances of successfully generating useful hybridoma lines is high. However, most projects enter the hybridoma generation stage after about 12-16 weeks from receipt of the protein.
What we need
Protein targets: To begin the project we will prefer 1 mg of protein soluble in a non-toxic buffer at a concentration of 0.5 mg/ml (preferably 1mg/ml). Before we enter the hybridoma generation at least another 0.5-1 mg is often required. However, lower amounts of antigen can often be accommodated. We can also assist you with production and purification of the necessary protein(s). Click here to find out more about our protein services.
Peptide targets: Due to the increased chances of success, we typically recommend utilizing protein antigens when possible. However, in cases where this is not scientifically or practically possible, synthesized peptides are a suitable option. VAPR can assist with both the design and generation of highly antigenic peptides.
Other approaches: VAPR also has experiences with cell-based immunizations, haptens / small molecules, and anti-idiotype antibody projects.
Someone from VAPR will follow up with you directly to further define project goals and parameters.