{"id":146,"date":"2023-02-20T20:49:02","date_gmt":"2023-02-20T20:49:02","guid":{"rendered":"https:\/\/medschool.prd.vanderbilt.edu\/vcmr2026\/?page_id=146"},"modified":"2026-02-19T21:29:10","modified_gmt":"2026-02-19T21:29:10","slug":"thaw-sperm-in-vials","status":"publish","type":"page","link":"https:\/\/medschool.vanderbilt.edu\/vcmr\/recovery-protocols\/thaw-sperm-in-vials\/","title":{"rendered":"Thaw Sperm in Vials"},"content":{"rendered":"\n\n

Reagents and supplies<\/h2>\n \n\n\n\n\t\n\n\t\n\t\n\t\n\t\n\t\n\t
Reagent<\/th>Source<\/th>Catalog Number<\/th>\n<\/tr>\n<\/thead>\n
Wide bore pipette tips<\/td>VWR<\/td>76635-646<\/td>\n<\/tr>\n
P-200 Pipetman<\/td>Rainin<\/td>P-200<\/td>\n<\/tr>\n
35 mm tissue culture dish<\/td>Fisher<\/td>0877230<\/td>\n<\/tr>\n
EmbryoMax mineral oil<\/td>Sigma<\/td>ES-005-C<\/td>\n<\/tr>\n
FERTIUP from CARD media kit<\/td>CosmoBio<\/td>KYD-004-EX-X5<\/td>\n<\/tr>\n
Beaker of 37\u00b0C water<\/td><\/td><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n \n

Prepare culture dishes (two dishes per vial)<\/h2>\n
  1. Prepare one 35 mm dish with one 90 \u03bcl drop of FERTIUP per dish covered in mineral oil.<\/li>
  2. Incubate for 30 minutes prior to use.<\/li><\/ol>\n \n

    Thawing sperm in vials<\/h2>\n
    1. Remove cryovial from LN2 and thaw at room temperature until ice is melted then place in 37\u00b0C water for 10 minutes.<\/li>
    2. Using a wide bore pipette tip, place one half of sperm into each FERTIUP dish. There is usually about 200 \u03bcl of sperm in a vial.<\/li><\/ol>\n \n\n","protected":false},"excerpt":{"rendered":"

      Reagents and supplies Prepare culture dishes (two dishes per vial) Prepare one 35 mm dish with one 90 \u03bcl drop of FERTIUP per dish covered in mineral oil. Incubate for 30 minutes prior to use. Thawing sperm in vials Remove cryovial from LN2 and thaw at room temperature until ice is melted then place in […]<\/p>\n","protected":false},"author":314,"featured_media":0,"parent":114,"menu_order":5,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"tags":[18,25,19,22],"class_list":["post-146","page","type-page","status-publish","hentry","tag-cryovials","tag-sperm","tag-thawing","tag-vials"],"acf":[],"ACF":{"topper_style":"default","topper_overline":"","topper_title":"","topper_intro":"","default_topper_background_type":"none","footer_style":"standard","recirculation_style":"none","page_style_option":"details","page_theme_option":"light","page_class_name":"","page_jumplinks_option":"yes","nav_menu":"","nav_menu_secondary":"no"},"_links":{"self":[{"href":"https:\/\/medschool.vanderbilt.edu\/vcmr\/wp-json\/wp\/v2\/pages\/146","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/medschool.vanderbilt.edu\/vcmr\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/medschool.vanderbilt.edu\/vcmr\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/medschool.vanderbilt.edu\/vcmr\/wp-json\/wp\/v2\/users\/314"}],"replies":[{"embeddable":true,"href":"https:\/\/medschool.vanderbilt.edu\/vcmr\/wp-json\/wp\/v2\/comments?post=146"}],"version-history":[{"count":6,"href":"https:\/\/medschool.vanderbilt.edu\/vcmr\/wp-json\/wp\/v2\/pages\/146\/revisions"}],"predecessor-version":[{"id":899,"href":"https:\/\/medschool.vanderbilt.edu\/vcmr\/wp-json\/wp\/v2\/pages\/146\/revisions\/899"}],"up":[{"embeddable":true,"href":"https:\/\/medschool.vanderbilt.edu\/vcmr\/wp-json\/wp\/v2\/pages\/114"}],"wp:attachment":[{"href":"https:\/\/medschool.vanderbilt.edu\/vcmr\/wp-json\/wp\/v2\/media?parent=146"}],"wp:term":[{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/medschool.vanderbilt.edu\/vcmr\/wp-json\/wp\/v2\/tags?post=146"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}