This microscope is well suited for confocal fluorescent imaging of live cell/tissue preparations, viewing samples in culture dishes, culture plates (6 wells and greater), chamber slides that can be inverted and a variety of custom made (by user) chambers no larger than a standard culture plate. The live tissue chamber with atmosphere heater, stage heater, humidity and CO2 control permit long-term time-lapse imaging for a wide variety of applications.

Imaging Modes:

• Laser scanning fluorescence and DIC (Nomarski)
• 3-D “Z-series”
• Time-series
• Tile scans (mosaics)
• Co-localization, FRET, FRAP, linear unmixing
• Multi-position

FLUORESCENCE EXCITATION/EMISSION

• Long UV (405 nm for dyes such as DAPI, Hoechst 346, fluorogold)
• Blue/Green (458,488, 514nm for blue, green, yellow dyes,
  e.g., CFP,YFP,GFP, Cy-2, FITC-like dyes)
• Green/Red (561nm for Cy-3, Texas Red, or rhodamine-like dyes)
• Red/Far-Red (633 nm for far-red dyes like Cy-5)
• Emission bandwidth for all dyes/laser lines is continuously variable providing huge flexibility for collecting images of unusual dyes and for minimizing bleed-through between colors.

Objective Lenses:

• 5x /.25 Fluor
• 10x / 0.50 Fluar
• 20x / 0.50 Plan Neofluar (long working distance)
• 20x / 0.80 Plan-Apochromat
• 40x / 1.10 LD C-Apochromat WATER
• 63x / 1.40 Plan-APOCHROMAT OIL

Charges:

Note: To use the microscope you must have an active iLab Account. Reservations are made through our on-line iLab calendar. Contact Tegy, or Jenny to arrange training.

Location

• LH 704

Download / View Quickstart Guide.