Why Use This Microscope?
The Nikon STORM is a super-resolution fluorescent microscope used for imaging structures that are too small to be resolved via conventional microscopy. Resolutions of 20 nm in XY and 50 nm in Z can be achieved using this microscope. Special buffers and dyes are required when using this microscope. Interested users are encouraged to research papers utilizing STORM imaging to ensure that this technology is right for their application.The following link is a good place to start for basic STORM imaging information .

Camera:

• Hamamatsu ORCA Flash 4.0 CMOS monochrome camera
• Nikon DS-Ri2 color camera

Imaging Modes:

• Stochastic Optical Reconstruction Microscopy (STORM)
• Conventional fluorescence microscope
• Chromogenic microscopy (DAB, H&E, etc)
• Multi-position (x/y)
• Tile scans
• High-speed time-lapse

Excitation Specs:

• STORM Diode Laser Lines:
  • 405 nm (DAPI), 20mW
  • 488 nm (FITC. GFP), 50mW
  • 561 nm (TRITC, Cy3, mCherry), 50mW
  • 647 nm (far red, Cy5), 125mW
• Lumencor SpectraIII LED light engine:
  • 365 nm (DAPI)
  • 440 nm (CFP)
  • 488 nm (FITC. GFP)
  • 514 nm (YFP)
  • 561 nm (TRITC, Cy3)
  • 594 nm (mCherry)
  • 640 nm (far red, Cy5)
  • 730 nm (near IR, Cy7)

Objective Lenses:

• 4x Plan Apo D 0.2 NA WD 20mm
• 10x Plan Apo 0.5 NA WD 4mm
• 20x Plan Apo 0.8 NA WD 1mm
• 100xH HP Apo TIRF 1.5 NA WD 120um Oil

Charges:

Note: To use the microscope you must have an active iLab account. Reservations are made through our on-line iLab calendar.Contact Sean to arrange training.

Location:

• 704A Light Hall