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The Vanderbilt Center for
Extracellular Vesicle Research

Synaptobrevin-2 dependent regulation of single synaptic vesicle endocytosis

AUTHORS

Chanaday Natali , Kavalali Ege , . . 2021 6 30; ().

ABSTRACT

Evidence from multiple systems indicates that vesicle SNARE (Soluble NSF Attachment REceptor) proteins are involved in synaptic vesicle endocytosis, although their exact action at the level of single vesicles are unknown. Here we interrogate the role of the main synaptic vesicle SNARE mediating fusion, synaptobrevin-2 (also called VAMP2), in modulation of single synaptic vesicle retrieval. We report that in the absence of synaptobrevin-2 fast and slow modes of single synaptic vesicle retrieval are impaired, indicating a role of the SNARE machinery in coupling exocytosis to endocytosis of single synaptic vesicles. Ultrafast endocytosis was impervious to changes in the levels of synaptobrevin-2, pointing to a separate molecular mechanism underlying this type of recycling. Taken together with earlier studies suggesting a role of synaptobrevin-2 in endocytosis, these results indicate that the machinery for fast synchronous release couples fusion to retrieval and regulates the kinetics of endocytosis in Ca2+ dependent manner.