Mark Magnuson
Department of Molecular Physiology and Biophysics (MPB)
Department of Cell and Developmental Biology (CDB)
Vanderbilt Center for Stem Cell Biology (VCSCB)
Program in Developmental Biology (PDB)
Vanderbilt Diabetes Research and Training Center (DRTC)
Vanderbilt-Ingram Cancer Center (VICC)
Beta Cell Interest Group (BIG)
Stem and Progenitor Cell Interest Group (SPRING)
The Magnuson Laboratory uses genetically-altered mice, next generation sequencing, and bioinformatics to study the gene regulatory network (GRN) that underpins pancreatic endocrine cell identity and function. Our current studies are focused on analyzing the effects of a chronic elevation in intracellular Ca2+ (excitotoxicity) on the GRN in mature beta-cells, and the role of several zinc finger transcription factors in these networks. In addition, we are interested in determining how Sox17 gene expression is controlled during early development, and are using CRISPR mutagenesis and embryonic stem cells to identify the key transcriptional factors that regulate formation of both the definitive endoderm and vascular endothelial cells.
The Magnuson Laboratory uses genetically-altered mice, next generation sequencing, and bioinformatics to study the gene regulatory network (GRN) that underpins pancreatic endocrine cell identity and function. Our current studies are focused on analyzing the effects of a chronic elevation in intracellular Ca2+ (excitotoxicity) on the GRN in mature beta-cells, and the role of several zinc finger transcription factors in these networks. In addition, we are interested in determining how Sox17 gene expression is controlled during early development, and are using CRISPR mutagenesis and embryonic stem cells to identify the key transcriptional factors that regulate formation of both the definitive endoderm and vascular endothelial cells.
Keywords: Stem cells , CRISPR , Zinc finger proteins , Gene networks , Bioinformatics , Diabetes
Research Area: Diabetes, Obesity, Metabolism , Stem Cell Biology & Regeneration , Gene Regulation , Genomics , Vascular Biology , Developmental Biology