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High Throughput Screening Facility

Lab Policy

What about the availability of compounds?
Our HTS facility has a small-molecule library of over 160,000 selected ‘drug-like’ compounds, primarily from ChemBridge and ChemDiv. We are working to increase our library through the efforts of the Chemical Synthesis Core, as well as other collaborators. Along with the Vanderbilt library, we also house a 100,000 compound library through the Molecular Libraries Screening Center Network (MLSCN) and several smaller libraries 200-2,000 compounds that include the SPECTRUM collection, NIH clinical collection, biolipids, and kinase inhibitor collections. For a list of these libraries please see our Compound libraries page.

What instrumentation is available?
Our instrumentation incorporates the leading edge of biomechanical technology. The crux of our lab technology is comprised of the following: Hamamatsu FDSS, Molecular Devices ImageXpress Micro XL, Perkin Elmer Envision 2100 and Enspire 2300, Spectramax M5, TopCount NXT, Labcyte Echo 555/555 OMICs, Thermo Electron Multidrop plate dispenser, Bio-Tek ELx plate washer, Agilent VPrep liquid handler, Agilent Bravo liquid handler, and more. For a complete list, please see the Instrumentation list in our technology section.

Who is eligible to use the facilities?
Users that pass criteria for assay design (reviewed by an internal advisory board), proof of funding support, necessary training, and user/password eligibility. Please see the Services section for examples offered thus far.

What types of experiments can be performed?
Our facility specializes in cellular-based assays as well as in vitro molecular assays, particularly G-protein coupled receptors (GPCRs), ion channels, disease targets, microbial targets, viral targets, and cancer targets. However, we are able to handle a wide variety of experimental setups, including cytotoxicity, enzyme reactions, immunochemistry, imaging, genomic screening, RNAi screening, and some DMPK assays. For a full discussion of our facility services please reference Assays Design and Assay Targets.