‘Resources and Instruments’
Cryo-Electron Microscopy
Feb. 7, 2024—Microscopes Titan Krios G4, 300kV TEM with Gatan K3 BioQuantum and ThermoScientific Falcon3 direct electron detectors: for collecting large dataset of high resolution cryo single particle and tomography data Glacios, 200kV TEM with Falcon4 direct electron detector: for screening vitrified grids and collecting small data sets of high resolution cryo single particle and tomography data...
Nuclear Magnetic Resonance
Feb. 7, 2024—• Bruker 500 MHz, two 600 MHz, an 800 MHz, and a 900 MHz spectrometers dedicated to the characterization of biomolecular systems. • Bruker 300 MHz, two 400 MHz, and 500 MHz spectrometers dedicated to small molecule characterization. • Bruker 600 MHz spectrometer equipped with LC-NMR and cryo-flow for high-throughput and metabonomics. For more...
Nikon N-STORM super-resolution microscope
Feb. 7, 2024—• Andor sCMOS camera at 256×256 for 2D and 3D-STORM • Lasers: 405, 488, 561, and 647 • 100x Apo TIRF 1.49/NA oil • Resolutions of 20 nm in XY and 50 nm in Z can be reached with this microscope
Electron Paramagnetic Resonance
Feb. 7, 2024—•Bruker BioSpin’s EPR CW-ELEXSYS 1-34GHz
Macromolecular X-ray Crystallography
Feb. 7, 2024—• Bruker Microstar microfocus rotating-anode X-ray generator with two Bruker Proteum PT135 CCD area detectors, on kappa goniometers. • Oxford Diffraction XcaliburPX Ultra with an ONYX CCD detector. • Auto-crystallization system based on a Hamilton Starlet liquid handler robot and a Mosquito nL dropsetter. • Access to synchrotron resources through membership in the Southeastern Regional...
Computational Structural Biology
Feb. 7, 2024—• Open-access molecular graphics laboratory equipped with several workstations that have access to over 80 molecular modeling, visualization, and analysis software applications. • 3500 Processor Cluster in the Advanced Computing Center for Research & Education.
Cell Imaging Shared Resource
Feb. 7, 2024—• LSM 510 Inverted, LSM 510 META Inverted, LSM 510 META Upright, and Olympus FV-1000 Inverted confocal microscopes. • Phillips CM-12 transmission electron microscope. • Leica DM IRB and Axiophot wide-field microscopes. • Image processing and rendering
Biophysical Characterization Core
Feb. 7, 2024—• analytical ultracentrifugation • circular dichroism • isothermal titration • differential scanning microcalorimetry
Automated Protein Crystallography
Feb. 7, 2024—• Mosquito Robot for high-throughput crystallization trials. • Xantus LCP Robot for high-throughput membrane protein crystallization trials. • Hamilton MICROLAB STARlet robot. • Formulatrix Rock Imager: a robust, easy-to-use, automated imaging system for protein crystallization.
AppliedPrecision OMX Super-resolution Microscope
Feb. 7, 2024—• sCMOS camera at 512×512 for 3D-SIM • Lasers: 405, 488, 568, and 642 • 60x Plan-Apochromat N/1.42 NA oil • Resolutions of 120 nm in XY and 300 nm in Z can be reached with this microscope.