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Resources & Instruments

Cryo-Electron Microscopy

Apr. 11, 2017—100 kV FEI Morgagni 200 kV FEI Technai 300 kV FEI Polara with K2 Summit DED camera FEI Vitrobot

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AppliedPrecision OMX Super-resolution Microscope

Jan. 29, 2016—• sCMOS camera at 512×512 for 3D-SIM​ • Lasers: 405, 488, 568, and 642  • 60x Plan-Apochromat N/1.42 NA oil • Resolutions of 120 nm in XY and 300 nm in Z can be reached with this microscope.

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Automated Protein Crystallography

Jan. 29, 2016—• Mosquito Robot for high-throughput crystallization trials. • Xantus LCP Robot for high-throughput membrane protein crystallization trials. • Hamilton MICROLAB STARlet robot. • Formulatrix Rock Imager: a robust, easy-to-use, automated imaging system for protein crystallization.

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Biophysical Characterization Core

Jan. 29, 2016—• analytical ultracentrifugation • circular dichroism • isothermal titration • differential scanning microcalorimetry

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Cell Imaging Shared Resource

Jan. 29, 2016—• LSM 510 Inverted, LSM 510 META Inverted, LSM  510 META Upright, and Olympus FV-1000 Inverted confocal microscopes. • Phillips CM-12 transmission electron microscope. • Leica DM IRB and Axiophot wide-field microscopes. • Image processing and rendering

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Computational Structural Biology

Jan. 29, 2016—• Open-access molecular graphics laboratory equipped with several workstations that have access to over 80 molecular modeling, visualization, and analysis software applications. • 3500 Processor Cluster in the Advanced Computing Center for Research & Education.

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Cryo-Electron Microscopy

Jan. 29, 2016—• FEI Tecnai T12 cryo-transmission electron microscope • FEI Tecnai F20 cryo-transmission electron microscope • FEI Tecnai TF30 Polara cryo-transmission electron microscope • FEI Vitrobot: Climate-controlled robotic grid preparation

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Macromolecular X-ray Crystallography

Jan. 29, 2016—• Bruker Microstar microfocus rotating-anode X-ray generator with two Bruker Proteum PT135 CCD area detectors, on kappa goniometers. • Oxford Diffraction XcaliburPX Ultra with an ONYX CCD detector. • Auto-crystallization system based on a Hamilton Starlet liquid handler robot and a Mosquito nL dropsetter. • Access to synchrotron resources through membership in the Southeastern Regional...

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Electron Paramagnetic Resonance

Jan. 29, 2016—•Bruker BioSpin’s EPR CW-ELEXSYS 1-34GHz

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Nikon N-STORM super-resolution microscope

Jan. 29, 2016—• Andor sCMOS camera at 256×256 for 2D and 3D-STORM • Lasers: 405, 488, 561, and 647 • 100x Apo TIRF 1.49/NA oil • Resolutions of 20 nm in XY and 50 nm in Z can be reached with this microscope

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Nuclear Magnetic Resonance

Jan. 29, 2016—• Bruker 500 MHz, two 600 MHz, an 800 MHz, and a 900 MHz spectrometers dedicated to the characterization of biomolecular systems. • Bruker 300 MHz, two 400 MHz, and 500 MHz spectrometers dedicated to small molecule characterization. • Bruker 600 MHz spectrometer equipped with LC-NMR and cryo-flow for high-throughput and metabonomics.

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