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Thaw Sperm in Vials

Reagents, supplies, and equipment:
  • Wide bore pipette tips
Thawing Sperm in Vials:
  1. Remove the cryovial from LN2 storage. Place the vial at room temperature until thawed, about 12 to 15 minutes.
  2. Using a wide bore pipette tip, remove the quantity of sperm needed for IVF.