The ASPIRE Path in Molecular Medicine

My thesis research focuses on host-virus interactions, specifically the molecular and structural characterization of the binding between viral nucleoprotein (NP) and the host mRNA export factor UAP56. Using biochemical assays and cryo-electron microscopy, I aim to identify the functional domains and amino acid residues involved and resolve its structural details. UAP56 is a human RNA helicase essential for mRNA splicing and export. During viral infection, it is hijacked to facilitate viral mRNA processing through interaction with viral nucleoprotein (NP). NP is a critical viral protein that maintains its genome integrity and supports its transcription and replication. Due to its highly conserved structure, NP is a promising target for antiviral drug development. My project primarily investigates NP in the context of influenza viruses, which remain a major public health threat because of their high transmissibility and seasonal outbreaks causing significant morbidity and mortality. To broaden the impact of our findings, we’ll evaluate how disrupting the UAP56-NP interaction affects viral replication in vivo with our collaborators at the UT Southwestern Medical Center. Our work bridges molecular virology and therapeutic development, with strong translational potential. Through APMM, I hope to incorporate clinical mentorship and explore the implications of this research for antiviral strategies.