Melting the Eis: non-detection of kanamycin resistance markers by routine diagnostic tests and identification of new -promoter variants
AUTHORS
Ley
SD ,
Pillay
S ,
Streicher
EM ,
van der Heijden
YF ,
Sirgel
F ,
Derendinger
B ,
de Kock
M ,
Gagneux
S ,
Warren
RM ,
Theron
G ,
de Vos
M ,
. Antimicrobial agents and chemotherapy. 2021 4 26; ().
- PMID: 33903113 [PubMed].
ABSTRACT
-promoter mutations can confer reduced kanamycin susceptibility. GenoType MTBDR a widely used assay evaluating this region, wrongly classified 17/410 isolates as -promoter wildtype. 6/17 isolates harbored mutations known to confer kanamycin resistance, and the remainder harbored either novel -promoter mutations (7/11) or disputed mutations (4/11). GenoType MTBDR can miss established and new variants that cause reduced susceptibility. These data highlight the importance of reflex phenotypic kanamycin testing.
Tags: alumni publications 2021