The use of fluorescently-tagged apoptolidins in cellular uptake and response studies.
AUTHORS
Chong
KM ,
Leelatian
N ,
Deguire
SM ,
Brockman
AA ,
Earl
D ,
Ihrie
RA ,
Irish
JM ,
Bachmann
BO ,
Sulikowski
GA ,
. The Journal of antibiotics. 2016 4 ; 69(4). 327-30
- PMID: 26956792 [PubMed].
ABSTRACT
The apoptolidins are glycomacrolide microbial metabolites reported to be selectively cytotoxic against tumor cells. Using fluorescently tagged active derivatives we demonstrate selective uptake of these four tagged glycomacrolides in cancer cells over healthy human blood cells. We also demonstrate the utility of these five fluorescently tagged glycomacrolides in fluorescent flow cytometry to monitor cellular uptake of the six glycomacrolides and cellular response.
Tags: 2016