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Determinants of raft partitioning of thepore-forming toxin VacA.


AUTHORS

Raghunathan K , Foegeding NJ , Campbell AM , Cover TL , Ohi MD , Kenworthy AK , . Infection and immunity. 2018 3 12; ().

ABSTRACT

, a Gram-negative bacterium, is a well-known risk factor for gastric cancer.vacuolating cytotoxin A (VacA) is a secreted pore-forming toxin that induces a wide range of cellular responses. Like many other bacterial toxins, VacA has been hypothesized to utilize lipid rafts to gain entry into host cells. Here, we use Giant Plasma Membrane Vesicles (GPMVs) as a model system to understand the preferential partitioning of VacA into lipid rafts. We show that a wild-type toxin predominantly associates with the raft phase. Acid activation of VacA enhances binding of the toxin to GPMVs but is not required for raft partitioning. VacA mutant proteins with alterations at the amino-terminus (resulting in impaired membrane channel formation) and a non-oligomerizing VacA mutant protein retain the ability to preferentially associate with lipid rafts. Consistent with these results, the isolated VacA p55 domain was capable of binding to lipid rafts. We conclude that the affinity of VacA for rafts is independent of its capacity to oligomerize or form membrane channels.



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