High-throughput drug screening of fine-needle aspiration-derived cancer organoids
AUTHORS
Bergdorf
K ,
Phifer
C ,
Bharti
V ,
Westover
D ,
Bauer
J ,
Vilgelm
A ,
Lee
E ,
Weiss
V ,
. STAR protocols. 2020 12 16; 1(3). 100212
- PMID: 33377106 [PubMed].
ABSTRACT
Generation of fine-needle aspiration (FNA)-derived cancer organoids has allowed us to develop a number of downstream applications. In this protocol, we start with organoids cultured in a semi-solid format. We dissociate organoids into single cells and then plate in a 384-well format for high-throughput drug screening. While this method must be fine-tuned for each individual organoid culture, it offers a format well suited for rapidly screening medium-sized drug/compound libraries (500-5,000 molecules) and generating dose-response curves to measure relative efficacy. For complete details on the use and execution of this protocol, please refer to Lee et al. (2020) and Vilgelm et al. (2020).
Tags: Publications