Cryo-Electron Microscopy

Posted by daviskd2 on Wednesday, February 7, 2024 in Instruments, Uncategorized .

Microscopes

• Titan Krios G4, 300kV TEM with Gatan K3 BioQuantum and ThermoScientific Falcon3 direct electron detectors: for collecting large dataset of high resolution cryo single particle and tomography data
• Glacios, 200kV TEM with Falcon4 direct electron detector: for screening vitrified grids and collecting small data sets of high resolution cryo single particle and tomography data

  

• TF20, 200kV TEM with 4k x 4k CCD: for collecting negative stain data and screening vitrified grid conditions

  

• Morgagni, 100kV TEM with 1k x 1k CCD: for screening and collecting negative stain data

Auxiliary

• 2 Vitrobots: Mark II and Mark IV
• 2 Gatan 626 cryo-transfer holders
• 2 Carbon evaporators
• 2 Glow dischargers
• GPU workstations

For more information: visit the Cryo-EM facility webpage on the Center for Structural Biology website.

Tags: Resources and Instruments