Genome Modified Mice
CRISPR Gene Editing
The Vanderbilt Genome Editing Resource has generated more than 150 mouse strains containing engineered deletions, point mutations, epitope tags, conditional knockouts, fluorescent reporters, and other types of alleles using CRISPR/Cas9.
CRISPR services include:
- Project consultation for grant applications and genome editing projects
- Allele design
- CRISPR reagent sourcing and testing in vitro and mouse embryos
- Embryo microinjection or electroporation
- Screening of founder mice
- Breeding of founders to test for germline transmission
- Sequencing of N1 heterozygous animals to verify allele fidelity
- Additional quality control assays as required for your allele
- Submission of allele for MGI curation and assignment of allele nomenclature and identification number
- Development of an automated genotyping assay in conjunction with TransnetYX
- Downstream support for manuscript preparation
Transgenic services include:
- Construct design
- DNA quality control and injection solution preparation
- Embryo microinjection
- Screening of founder mice
- Breeding of founders to test for germline transmission
- Assessment of copy number
- Inverse PCR mapping
- Submission of allele for MGI curation and assignment of allele nomenclature and identification number
- Development of an automated genotyping assay in conjunction with TransnetYX
- Downstream support for manuscript preparation
We can help you test and optimize other types of genome-editing and transgenic technologies under collaborative agreements. We are experienced in the use of piggyBac transposase and CRISPR interference technologies in mice. We can also produce genome-edited human induced pluripotent stem cells.