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Post-doc Publication: biallelic disruption in zebrafish
Nov. 15, 2013— Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system. Jao LE, Wente SR, Chen W. Source Department of Cell and Developmental Biology and Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232-8240. Abstract A simple and robust method for targeted mutagenesis in zebrafish has...
Post-doc Publication: CRISPR/Cas9 method in zebrafish
Aug. 14, 2013—A simple and robust method for targeted mutagenesis in zebrafish has long been sought. Previous methods generate monoallelic mutations in the germ line of F0 animals, usually delaying homozygosity for the mutation to the F2 generation. Generation of robust biallelic mutations in the F0 would allow for phenotypic analysis directly in injected animals. Recently the...
Lab Publication: Insights into the mitotic phosphoproteome
Jun. 3, 2013—Mol Cell Proteomics. 2013 May;12(5):1074-86. doi: 10.1074/mcp.M112.025924. Epub 2013 Jan 7. Comprehensive proteomics analysis reveals new substrates and regulators of the fission yeast clp1/cdc14 phosphatase. Chen JS, Broadus MR, McLean JR, Feoktistova A, Ren L, Gould KL. ‡Howard Hughes Medical Institute and Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, 1161...
Publication: Scribble implicated in control of vesicle traffic
Apr. 4, 2013—The Scribble polarity protein stabilizes E-cadherin/p120-catenin binding and blocks retrieval of E-cadherin to the Golgi. Lohia M, Qin Y, Macara IG. Source Department of Microbiology, Ctr for Cell Signaling, University of Virginia School of Medicine, Charlottesville, VA, USA. Abstract Several polarity proteins, including Scribble (Scrb) have been implicated in control of vesicle traffic, and in...
Student Publication: Asunder promotes dynein recruitment
Apr. 3, 2013—Jodoin JN, Shboul M, Sitaram P, Zein-Sabatto H, Reversade B, Lee E, Lee LA. Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37232-8240, USA. Abstract Recruitment of dynein motors to the nuclear surface is an essential step for nucleus-centrosome coupling in prophase. In cultured human cells, this dynein pool is anchored...