2018 CDB Retreat Movie Submissions
First Place Winners
Nilay Taneja (Burnette Lab)
Alpha_Actinin2
A human cardiomyocyte derived from induced pluripotent stem cells, expressing the protein alpha actinin that marks the Z-discs of sarcomeres, the fundamental unit of contraction in the heart. The movie shows a cardiomyocyte forming the Z-discs of sarcomeres over a time period of 36 hours.
Meredith Weck (Tyska Lab)
WeckPCDH24-mCherry-FRB EGFP-Myo10noCC-FKBP HeLa_001_crop-MaxIP-aligned-120
Additional Movie Submissions
Adian Fenix (Burnette Lab)
Beating Cardiac Microtissue
Cardiac microtissue differentiated from human induced pluripotent stem cells. Cardiac microtissue displays spontaneous beating in culture as soon as 12 days post differentiation (shown here). Notice coordinated beating of cells and deformation of the soft matrigel matress. In the human body, the heart must beat efficiently billions of times throughout the average lifespan. It has taken decades of tinkering to engineer this type of beating in vitro while maintaining cell health. Image Credit: Aidan Fenix, Burnette Lab
Keyada Frye (Kaverina Lab)
Keyada Frye (Kaverina Lab)
Cell division of RPE1 cell expressing GFP-centrin (green) and RFP-TGN (red). Cells were treated with Centrinone B for 72 hours to deplete the centrosomes. Daughter cells' post-mitotic Golgi compaction varies depending on absence or presence of a centrosome . MAX z-projection, 100x.
Sun Wook-Kim (Lau Lab)
Shear stress induces vacuole formation in dense Caco-2 BBE monolayers.
Leslie Meenderink (Tyska Lab)
Live Cell Spinning Disk Confocal
Motile microvilli on the apical aspect of LLC-PK1-CL4 cell early in differentiation demonstrating that immature microvilli are highly dynamic extending perpedicular from the cell surface and translocating across the cell surface both as individual protrusions and grouped clusters. Scale indicated on the image. Color coding indicates depth in Z ranging from 0 to 6 um (legend at left).
Megan Postema (Tyska Lab)
Spinning Disk confocal imaging of an induced Ls174T-W4 cell expressing mCherry-Utrophin (magenta) and EGFP-IRTKS (green). Movie was acquired every 10 seconds for 30 minutes and is played at 20 frames per second. Scale bar is 5 μm. Image credit: Meagan Postema, Tyska Lab