2019 CDB Retreat Movie Submissions
First Place Winner
Leslie Meenderink
“Clustering Microvilli”
CL4 cells expressing mCherry-Espin to mark F-actin in microvilli show individual microvilli forming centrally then migrating to the cell edges where they are stabilized in large clusters. Movie 12 hours.
Additional Movie Submissions
Veronica Farmer
“XMAP215 Induces Microtubule Curling”
Microtubule (red) growing in vitro with EB1 (green) and XMAP215.
Beth Lawrence
“Human SSNA1 Promotes Microtubule Curvature”
SSNA1 (Sjogren’s Syndrome Nuclear Autoantigen 1) is a coiled-coil protein that self-assembles into fibrils and localizes to microtubule-based structures such as cilia and flagella. In vitro, purified SSNA1 localizes to and stabilizes dynamic microtubules and causes them to curl. Images were acquired by TIRF microscopy at 0.2 fps. GMPCPP-stabilized.
Natalya Ortolano
“Accidental Apoptosis”
Human pluripotent stem cells expressing FUCCI constructs (red=G1 phase, green=G2/M or S phase) were imaged overnight. However, the incubator was not correctly set up for the cells, so the cells can be seen apoptosing overtime.
Megan Rasmussen
Maze of Mitochondria
Three-dimensional reconstruction of mitochondria in a brain microvascular endothelial cell (BMEC). Cyan/green = mitochondria (stained with MitoTracker Red CMXRos). Image aquired on Nikon SIM (100X).
Anneke Sanders
“Microtubule Nucleation in a Human Retinal Pigment Epithelial Cell”
A new microtubule network is formed after washout of nocodazole, a microtubule depolymerizing drug. Microtubules are labelled by Emerald-EB3 which binds to growing plus-ends. Recorded using a confocal spinning disk microscope.
Shwetha Narasimhan
“Contractile Actin Fiber Dynamics in a Fibroblast”
Contractile actin fiber dynamics seen in a lung fibroblast cell. Cell is expressing EGFP-Myosin Regulatory Light Chain. Video taken with a confocal spinning disk microscope. FPS=24. Data processed in Fiji to apply temporal color coding (Spectrum LUT) according to Z position.
Nilay Taneja
“Mitochondria in a Beating Cell”
Shown is a human cardiomyocyte expressing a mitochondrial marker beating over 15 seconds.