MSTPublications: January 2018
Congratulations to all of our MSTP students on their successful publications! Take a look at the great work our students are doing.
First Author Original Research:
Balikov DA, Crowder SW, Lee JB, Lee Y, Ko UH, Kang ML, Kim WS, Shin JH, Sung HJ.
Int J Mol Sci. 2018 Jan 25;19(2). pii: E359. doi: 10.3390/ijms19020359.
Human mesenchymal stem cells (hMSCs) have been widely studied for therapeutic development in tissue engineering and regenerative medicine. They can be harvested from human donors via tissue biopsies, such as bone marrow aspiration, and cultured to reach clinically relevant cell numbers. However, an unmet issue lies in the fact that the hMSC donors for regenerative therapies are more likely to be of advanced age. Their stem cells are not as potent compared to those of young donors, and continue to lose healthy, stemness-related activities when the hMSCs are serially passaged in tissue culture plates. Here, we have developed a cheap, scalable, and effective copolymer film to culture hMSCs obtained from aged human donors over several passages without loss of reactive oxygen species (ROS) handling or differentiation capacity. Assays of cell morphology, reactive oxygen species load, and differentiation potential demonstrate the effectiveness of copolymer culture on reduction in senescence-related activities of aging donor-derived hMSCs that could hinder the therapeutic potential of autologous stem cell therapies. (By Dan Balikov, M3)
Cis-regulatory evolution integrated the Bric-à-brac transcription factors into a novel fruit fly gene regulatory network.
Roeske MJ, Camino EM, Grover S, Rebeiz M, Williams TM.
Elife. 2018 Jan 3;7. pii: e32273. doi: 10.7554/eLife.32273.
Gene expression evolution through gene regulatory network (GRN) changes has gained recognition as a driver of morphological evolution. However, understanding how GRNs evolve is hampered by finding relevant cis-regulatory element (CRE) mutations, and interpreting the protein-DNA interactions they alter. We investigated evolutionary changes in duplicated transcription factors and a key target gene in a GRN underlying the novel dimorphic pigmentation of D. melanogaster and its relatives. Here, we show that an ancestral transcription factor gained a role in sculpting sex-specific pigmentation through the evolution of binding sites in a CRE. This work demonstrates how a new trait can evolve by incorporating existing transcription factors into a GRN through CRE evolution, an evolutionary path likely to predominate newly evolved functions of transcription factors. The majority of this work was completed for my undergraduate thesis before entering the Vanderbilt MSTP. Although my future work will be more translational, this evolutionary and developmental biology is what convinced me to venture into the world of science. (By Maxwell Roeske, M2)
Co-authorships, Clinical Studies, and Reviews:
Glucagon-like peptide 1 signaling inhibits allergen-induced lung IL-33 release and reduces group 2 innate lymphoid cell (ILC2) cytokine production in vivo.
Toki S, Goleniewska K, Reiss S, Zhang J, Bloodworth MH, Stier MT, Zhou W, Newcomb DC, Ware LB, Stanwood GD, Galli A, Boyd KL, Niswender KD, Peebles RS Jr.
J Allergy Clin Immunol. 2018 Jan 10. pii: S0091-6749(18)30027-7. doi: 10.1016/j.jaci.2017.11.043. [Epub ahead of print]
Established, emerging, and elusive molecular targets in the treatment of lung cancer.
Gallant JN, Lovly CM.
J Pathol. 2018 Jan 17. doi: 10.1002/path.5038. [Epub ahead of print] Review.
Murine Hepatitis Virus nsp14 Exoribonuclease Activity Is Required for Resistance to Innate Immunity.
Case JB, Li Y, Elliott R, Lu X, Graepel KW, Sexton NR, Smith EC, Weiss SR, Denison MR.
J Virol. 2017 Dec 14;92(1). pii: e01531-17. doi: 10.1128/JVI.01531-17. Print 2018 Jan 1.
Elevating adipose eosinophils in obese mice to physiologically normal levels does not rescue metabolic impairments.
Bolus WR, Peterson KR, Hubler MJ, Kennedy AJ, Gruen ML, Hasty AH.
Mol Metab. 2017 Dec 16. pii: S2212-8778(17)30898-0. doi: 10.1016/j.molmet.2017.12.004. [Epub ahead of print]
Allele-specific SHAPE-MaP assessment of the effects of somatic variation and protein binding on mRNA structure.
Lackey L, Coria A, Woods C, McArthur E, Laederach A.
RNA. 2018 Jan 9. pii: rna.064469.117. doi: 10.1261/rna.064469.117. [Epub ahead of print]
Hepatocyte estrogen receptor alpha mediates estrogen action to promote reverse cholesterol transport during Western-type diet feeding.
Zhu L, Shi J, Luu TN, Neuman JC, Trefts E, Yu S, Palmisano BT, Wasserman DH, Linton MF, Stafford JM.
Mol Metab. 2017 Dec 29. pii: S2212-8778(17)30683-X. doi: 10.1016/j.molmet.2017.12.012. [Epub ahead of print]
Rare Undiagnosed Primary Amyloidosis Unmasked During Surgical Treatment of Primary Hyperparathyroidism: A Case Report.
Gallagher KC, Geromes AB, Stokes J, Reddy IA, Lewis JS Jr., Baregamian N.
J Endocr Soc. 2018 Jan 3;2(2):112-116. doi: 10.1210/js.2017-00445. eCollection 2018 Feb 1.
Discovery of human cell selective effector molecules using single cell multiplexed activity metabolomics.
Earl DC, Ferrell PB Jr, Leelatian N, Froese JT, Reisman BJ, Irish JM, Bachmann BO.
Nat Commun. 2018 Jan 2;9(1):39. doi: 10.1038/s41467-017-02470-8.
Metabolic Barriers to T Cell Function in Tumors.
Sugiura A, Rathmell JC.
J Immunol. 2018 Jan 15;200(2):400-407. doi: 10.4049/jimmunol.1701041. Review.
Treatment of progressive herpes zoster-induced vasculopathy with surgical revascularization: effects on cerebral hemodynamics.
Lants SK, Watchmaker JM, Juttukonda MR, Davis LT, Donahue MJ, Fusco MR.
World Neurosurg. 2017 Dec 20. pii: S1878-8750(17)32203-9. doi: 10.1016/j.wneu.2017.12.087. [Epub ahead of print]
Cerebral hemodynamics and pseudo-continuous arterial spin labeling considerations in adults with sickle cell anemia.
Juttukonda MR, Jordan LC, Gindville MC, Davis LT, Watchmaker JM, Pruthi S, Donahue MJ.
NMR Biomed. 2017 Feb;30(2). doi: 10.1002/nbm.3681. Epub 2017 Jan 4.
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