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Post-doc Publication: CRISPR/Cas9 method in zebrafish

Aug. 14, 2013—A simple and robust method for targeted mutagenesis in zebrafish has long been sought. Previous methods generate monoallelic mutations in the germ line of F0 animals, usually delaying homozygosity for the mutation to the F2 generation. Generation of robust biallelic mutations in the F0 would allow for phenotypic analysis directly in injected animals. Recently the...

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Faculty Appointment: Jason MacGurn, Ph.D.

Jul. 8, 2013—I am very pleased to announce the appointment of Jason MacGurn, Ph.D., to the rank of Assistant Professor of Cell and Developmental Biology on the tenure track, effective August 1, 2013. Jason is currently a Post Doctoral Fellow in Scott Emr’s Lab at Cornell University Weill Institute for Cell and Molecular Biology.  He was jointly...

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Lab Publication: Insights into the mitotic phosphoproteome

Jun. 3, 2013—Mol Cell Proteomics. 2013 May;12(5):1074-86. doi: 10.1074/mcp.M112.025924. Epub 2013 Jan 7. Comprehensive proteomics analysis reveals new substrates and regulators of the fission yeast clp1/cdc14 phosphatase. Chen JS, Broadus MR, McLean JR, Feoktistova A, Ren L, Gould KL.   ‡Howard Hughes Medical Institute and Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, 1161...

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Publication: Scribble implicated in control of vesicle traffic

Apr. 4, 2013—The Scribble polarity protein stabilizes E-cadherin/p120-catenin binding and blocks retrieval of E-cadherin to the Golgi. Lohia M, Qin Y, Macara IG. Source Department of Microbiology, Ctr for Cell Signaling, University of Virginia School of Medicine, Charlottesville, VA, USA. Abstract Several polarity proteins, including Scribble (Scrb) have been implicated in control of vesicle traffic, and in...

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Student Publication: Asunder promotes dynein recruitment

Apr. 3, 2013—Jodoin JN, Shboul M, Sitaram P, Zein-Sabatto H, Reversade B, Lee E, Lee LA. Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37232-8240, USA. Abstract Recruitment of dynein motors to the nuclear surface is an essential step for nucleus-centrosome coupling in prophase. In cultured human cells, this dynein pool is anchored...

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Faculty Appointment: Ken Lau, Ph.D.

Mar. 21, 2013—Cell & Developmental Biology welcomes new faculty member Ken Lau, Ph.D. Dr. Ken Lau comes to Vanderbilt after completing his post postdoctorate training in the laboratory of Kenvi Hargis, Ph.D. In the Molecular Pathology Unit at the Massachusetts General Hospital and Harvard Medical School. Dr. Lau was chosen to join the Department of Cell and...

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CDB Announces Mary Heath as new Department Administrator

Mar. 21, 2013—Ian G. Macara, Ph.D. Louise B. McGavock and Chair, Department of Cell and Developmental Biology Vanderbilt University Medical School Mary Heath, MBA, CFA, CRA, will assume the position of Departmental Administrator in the Department of Cell and Developmental Biology on April 22, 2013. Ms. Heath will be making the transition from Boston to Nashville as...

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Morphological and neurochemical comparisons between pulvinar and V1 projections to V2

Jan. 22, 2013—Morphological and neurochemical comparisons between pulvinar and V1 projections to V2 Roan Marion1, Keji Li2, Gopathy Purushothaman3, Yaoguang Jiang2 andVivien A. Casagrande1 Article first published online: 22 JAN 2013 DOI: 10.1002/cne.23203 Abstract The flow of visual information is clear at the earliest stages: the retina provides the driving (main signature) activity for the lateral geniculate...

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Loss of the par3 polarity protein promotes breast tumorigenesis and metastasis.

Nov. 23, 2012—McCaffrey LM, Montalbano J, Mihai C, Macara IG. Rosalind and Morris Goodman Cancer Research Centre, Department of Oncology, McGill University, Montreal H3A 1A3, Canada. Electronic address: luke.mccaffrey@mcgill.ca. Abstract Loss of epithelial organization is a hallmark of carcinomas, but whether polarity regulates tumor growth and metastasis is poorly understood. To address this issue, we depleted the...

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Cytokinetic regulation of cell polarity

Nov. 23, 2012—The rod-shaped fission yeast Schizosaccharomyces pombe, which undergoes cycles of monopolar-to-bipolar tip growth, is an attractive organism for studying cell-cycle regulation of polarity establishment. While previous research has described factors mediating this process from interphase cell tips, we found that division site signaling also impacts the re-establishment of bipolar cell growth in the ensuing cell...

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Spatial regulation of a cell cycle phosphatase

Nov. 23, 2012—The rod-shaped fission yeast Schizosaccharomyces pombe, which undergoes cycles of monopolar-to-bipolar tip growth, is an attractive organism for studying cell-cycle regulation of polarity establishment. While previous research has described factors mediating this process from interphase cell tips, we found that division site signaling also impacts the re-establishment of bipolar cell growth in the ensuing cell...

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Multisite phosphoregulation of Cdc25 activity refines the mitotic entrance and exit switches Posted August 13th, 2012 by admin

Aug. 13, 2012—Lucy X. Lua, Maria Rosa Domingo-Sananesb, Malwina Huzarskaa, Bela Novakb, and Kathleen L. Gould a,c,1 + Author Affiliations a Department of Cell and Developmental Biology, and c The Howard Hughes Medical Institute, Vanderbilt University School of Medicine, Nashville, TN 37212; and b Oxford Centre for Integrative Systems Biology, Department of Biochemistry, University of Oxford, Oxford...

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Lis-1 and asunder regulate dynein

Aug. 13, 2012—oojitha Sitaram, Michael A. Anderson*, Jeanne N. Jodoin, Ethan Lee and Laura A. Lee‡ + Author Affiliations Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, U-4225 Medical Research Building III, 465 21st Avenue South, Nashville, TN 37232-8240, USA. + Author Notes * Present address: Georgetown University Law Center, 600 New Jersey Avenue...

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asymmetry for cytokinesis

May. 14, 2012—The fission yeast septation initiation network (SIN) kinase, Sid2, is required for SIN asymmetry and regulates the SIN scaffold, Cdc11 Anna Feoktistovaa, Jennifer Morrell-Falveya, Jun-Song Chena, N. Sadananda Singh, Mohan K. Balasubramanianb,c, and Kathleen L. Goulda,1 + Affiliations aHoward Hughes Medical Institute and Department of Cell and Developmental Biology, Vanderbilt University, Nashville, TN 37212 bTemasek...

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Identification of XIAP as a critical regulator of Wnt signaling

Mar. 21, 2012—beta-catenin accumulates in the nucleus and binds to the transcription factor, TCF/Lef, to activate Wnt target gene transcription. In order for beta-catenin to bind TCF/Lef, however, the transcriptional corepressor Groucho (Gro)/TLE, which is bound to TCF/Lef, must be displaced. Work by Hanson et al. shows that X-linked Inhibitor of Apoptosis (XIAP), an E3 ubiquitin ligase,...

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