Postdoctoral Associate – Bhaduri-McIntosh Lab, University of Florida, Gainesville, FL
Job description:
The Bhaduri-McIntosh lab at the University of Florida, U.S.A. is seeking a motivated post-doctoral candidate to pursue recently discovered novel components of the cellular DNA replication machinery driven by the cancer-causing Epstein-Barr virus. This research combines standard molecular biology, immunology, genetic, and biochemical approaches as well as cutting edge approaches such as DNA fiber analysis, mass spectrometry, and iPOND to investigate host-pathogen relationships in separated populations of latent and lytically infected cells and patient-derived cells. The Bhaduri-McIntosh lab provides a highly supportive environment with ample opportunity for collaboration. This is combined with rigorous mentoring to create an individualized development plan to achieve the career goals of each team member.
The ideal candidate will be highly motivated with a strong work ethic and demonstrated creativity in prior publications.
Duties:
- Design and develop research projects with the Principal Investigator
- Lead at least two independent research projects simultaneously alongside collaborative projects with other team members
- Conduct experiments using a variety of techniques including qPCR, RT-qPCR, chromatin immunoprecipitation, cloning, immunoprecipitation, immunofluorescence, Western blotting, FACS, virus mutagenesis, proximity ligation assays, iPOND, DNA molecular combing, mass spectrometry, transcriptomics, CRISPR-Cas9, etc. Perform data collection and analysis.
- Initiate and maintain cell cultures
- Prepare and maintain biological stocks
- Maintain proper lab and experimental records, both digital and hard copy
- Test, optimize, troubleshoot new and existing methods and techniques, and update protocols
- Guide and train undergraduate and graduate student researchers
- Prepare scientific results for publication in peer-reviewed journals and grant applications
- Maintain laboratory equipment and order supplies as needed
- Present results at internal and external meetings and scientific conferences
Minimum requirements:
- Ph.D. degree in the appropriate area of specialization. A strong interest in research, as well as excellent analytical, oral and written communication skills.
- First-author peer-reviewed publications in English language journals.
- Experience in mammalian cell culture and basic biology techniques including qPCR, RT-qPCR, chromatin immunoprecipitation, cloning, immunoprecipitation, fluorescence microscopy, and Western blotting.
- Experience in Virology, mass spectrometry, transcriptomics, CRISPR-Cas9, protein purification.